Protein, which is both involved in DNA repair and protein ubiquitination, as part of the UV-DDB complex and DCX complexes, respectively . Core component of the UV-DDB complex , a complex that recognizes UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway to initiate DNA repair . The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers , 6-4 photoproducts , apurinic sites and short mismatches . Also functions as a component of numerous distinct DCX E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins . The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1 . DCX may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage . The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair . DCX also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER . DCX plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication . DCX plays a role in transcription-coupled repair . The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1 . DDB1-mediated CRY1 degradation promotes FOXO1 protein stability and FOXO1-mediated gluconeogenesis in the liver . By acting on TET dioxygenses, essential for oocyte maintenance at the primordial follicle stage, hence essential for female fertility . Maternal factor required for proper zygotic genome activation and genome reprogramming .

DNA damage-binding protein 1 (DDB p127 subunit) (DNA damage-binding protein a) (DDBa) (Damage-specific DNA-binding protein 1) (HBV X-associated protein 1) (XAP-1) (UV-damaged DNA-binding factor) (UV-damaged DNA-binding protein 1) (UV-DDB 1) (XPE-binding factor) (XPE-BF) (Xeroderma pigmentosum group E-complementing protein) (XPCe)

q16531

Human,Mouse,Rat

WB,IHC,IF,ICC

WB 1:5000-1:50000
IHC 1:100-1:200
IF 1:100
ICC 1:100

~127 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.