Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins . As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme . The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 . The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component . DCX directs ubiquitination of JUN . DCX directs ubiquitination of XPC . DCX ubiquitinates histones H3-H4 and is required for efficient histone deposition during replication-coupled and replication-independent nucleosome assembly, probably by facilitating the transfer of H3 from ASF1A/ASF1B to other chaperones involved in histone deposition . DCX plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of p53/TP53 in response to radiation-induced DNA damage and during DNA replication . DCX directs autoubiquitination of DTL . In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip . Is involved in ubiquitination of HOXA9 . The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1 . The DCX complex plays a role in transcription-coupled repair . A number of DCX complexes are part of the DesCEND pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation . The DCX complex is a master regulator of the transition from G1 to S cell phase by mediating ubiquitination of phosphorylated cyclin-D . The DCX complex also acts as a regulator of Cul5-RING E3 ubiquitin-protein ligase complexes by mediating ubiquitination and degradation of Elongin-C component of CRL5 complexes . With CUL4B, contributes to ribosome biogenesis .

Cullin-4A (CUL-4A)

q13619

Human

WB,IHC,IP

WB 1:5000-1:50000
IHC 1:100-1:200
IP 1:50

~88 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.