Multifunctional transporter that transports L-glutamate as well as multiple ions such as chloride, proton, potassium, sodium and phosphate . At the synaptic vesicle membrane, mainly functions as an uniporter which transports preferentially L-glutamate but also phosphate from the cytoplasm into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells . The L-glutamate or phosphate uniporter activity is electrogenic and is driven by the proton electrochemical gradient, mainly by the electrical gradient established by the vacuolar H-ATPase across the synaptic vesicle membrane . In addition, functions as a chloride channel that allows a chloride permeation through the synaptic vesicle membrane that affects the proton electrochemical gradient and promotes synaptic vesicles acidification . Moreover, may function as a K/H antiport allowing to maintain the electrical gradient and to decrease chemical gradient and therefore sustain vesicular glutamate uptake . The vesicular K/H antiport activity is electroneutral . At the plasma membrane, following exocytosis, functions as a symporter of Na and phosphate from the extracellular space to the cytoplasm allowing synaptic phosphate homeostasis regulation . The symporter activity is driven by an inside negative membrane potential and is electrogenic . Is necessary for synaptic signaling of visual-evoked responses from photoreceptors .

Vesicular glutamate transporter 1 (VGluT1) (Brain-specific Na(+)-dependent inorganic phosphate cotransporter) (Solute carrier family 17 member 7)

q9p2u7

Human, Mouse, Rat

WB,IHC,IF,ICC,IP

WB 1:1000
IHC 1:1000
IF 1:200
ICC 1:200
IP 1:20

~62 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.