Protein kinase which plays an important role in mitotic cell cycle progression . Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis . Phosphorylates EML4 at 'Ser-146', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression . Phosphorylates RPS6KB1 . Acts as an essential activator of the NLRP3 inflammasome assembly independently of its kinase activity . Acts by unlocking NLRP3 following NLRP3 tranlocation into the microtubule organizing center , relieving NLRP3 autoinhibition and promoting formation of the NLRP3:PYCARD complex, and activation of CASP1 . Serves as a cellular switch that enforces mutual exclusivity of the inflammasome response and cell division: interaction with NEK9 prevents interaction with NLRP3 and activation of the inflammasome during mitosis .

Serine/threonine-protein kinase Nek7 (EC 2.7.11.34) (Never in mitosis A-related kinase 7) (NimA-related protein kinase 7)

q8tdx7

Human,Mouse,Rat

WB,IP

WB 1:1000-1:5000
IP 1:10-1:100

~35 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.