Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate P/PI4P) to form phosphatidylinositol 4,5-bisphosphate P2/PIP2), a lipid second messenger that regulates several cellular processes such as signal transduction, vesicle trafficking, actin cytoskeleton dynamics, cell adhesion, and cell motility . PtdInsP2 can directly act as a second messenger or can be utilized as a precursor to generate other second messengers: inositol 1,4,5-trisphosphate , diacylglycerol or phosphatidylinositol-3,4,5-trisphosphate P3/PIP3) . PIP5K1A-mediated phosphorylation of PtdInsP is the predominant pathway for PtdInsP2 synthesis . Together with PIP5K1A, is required for phagocytosis, both enzymes regulating different types of actin remodeling at sequential steps . Promotes particle attachment by generating the pool of PtdInsP2 that induces controlled actin depolymerization to facilitate Fc-gamma-R clustering. Mediates RAC1-dependent reorganization of actin filaments. Required for synaptic vesicle transport . Controls the plasma membrane pool of PtdInsP2 implicated in synaptic vesicle endocytosis and exocytosis . Plays a role in endocytosis mediated by clathrin and AP-2 . Required for clathrin-coated pits assembly at the synapse . Participates in cell junction assembly . Modulates adherens junctions formation by facilitating CDH1/cadherin trafficking . Required for focal adhesion dynamics . Modulates the targeting of talins to the plasma membrane and their efficient assembly into focal adhesions . Regulates the interaction between talins and beta-integrins . Required for uropodium formation and retraction of the cell rear during directed migration . Has a role in growth factor-stimulated directional cell migration and adhesion . Required for talin assembly into nascent adhesions forming at the leading edge toward the direction of the growth factor . Negative regulator of T-cell activation and adhesion . Negatively regulates integrin alpha-L/beta-2 polarization and adhesion induced by T-cell receptor . Together with PIP5K1A has a role during embryogenesis and together with PIP5K1B may have a role immediately after birth .

Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma (PIP5K1-gamma) (PtdIns(4)P-5-kinase 1 gamma) (EC 2.7.1.68) (Phosphatidylinositol 4-phosphate 5-kinase type I gamma) (PIP5KIgamma)

o70161

Human,Mouse,Rat

WB

WB 1:1000-1:5000

~73 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.