Histone demethylase that can demethylate both 'Lys-4' and 'Lys-9' of histone H3, thereby acting as a coactivator or a corepressor, depending on the context . Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed . Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- and di-methylated H3K4me . May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity . Also acts as a coactivator of androgen receptor -dependent transcription, by being recruited to AR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in AR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 , a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A . Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1 . Demethylates methylated 'Lys-42' and methylated 'Lys-117' of SOX2 . Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase recruitment, a number of genes implicated in multilineage blood cell development . Facilitates epithelial-to-mesenchymal transition by acting as an effector of SNAI1-mediated transcription repression of epithelial markers E-cadherin/CDH1, CDN7 and KRT8 . Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7 . Required for the repression of GIPR expression .

Lysine-specific histone demethylase 1A (EC 1.14.99.66) (BRAF35-HDAC complex protein BHC110) (Flavin-containing amine oxidase domain-containing protein 2) ([histone H3]-dimethyl-L-lysine(4) FAD-dependent demethylase 1A)

o60341

Human, Mouse, Rat

WB,IHC,IF,ICC,IP,FC

WB 1:2000-1:10000
IHC 1:50-1:200
IF 1:100-1:200
ICC 1:100-1:200
IP 1:20-1:50
FC 1:20

~93 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.