TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton . The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Binds and recruits PATJ to tight junctions where it connects and stabilizes apical and lateral components of tight junctions . Promotes cell-cycle progression through the sequestration of cyclin D1 at tight junctions during mitosis which prevents CCND1 degradation during M-phase and enables S-phase transition . With TJP1 and TJP2, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus . Contrary to TJP2, TJP3 is dispensable for individual viability, embryonic development, epithelial differentiation, and the establishment of TJs, at least in the laboratory environment .

Tight junction protein ZO-3 (Tight junction protein 3) (Zona occludens protein 3) (Zonula occludens protein 3)

o95049

Human,Rat

WB

WB 1:2000-1:20000

~102 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.