Transcription factor that acts as a master regulator of lysosomal biogenesis, autophagy, lysosomal exocytosis, lipid catabolism, energy metabolism and immune response . Specifically recognizes and binds E-box sequences ; efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF . Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, TFEB phosphorylation by MTOR promotes its cytosolic retention and subsequent inactivation . Upon starvation or lysosomal stress, inhibition of MTOR induces TFEB dephosphorylation, resulting in nuclear localization and transcription factor activity . Specifically recognizes and binds the CLEAR-box sequence present in the regulatory region of many lysosomal genes, leading to activate their expression, thereby playing a central role in expression of lysosomal genes . Regulates lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1 . Acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy . In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4 T-cells and thymus-dependent humoral immunity . Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer . Plays a role in the signal transduction processes required for normal vascularization of the placenta . Involved in the immune response to infection by the bacteria S.aureus, S.typhimurium or S.enterica: infection promotes itaconate production, leading to alkylation, resulting in nuclear localization and transcription factor activity . Itaconate-mediated alkylation activates TFEB-dependent lysosomal biogenesis, facilitating the bacteria clearance during the antibacterial innate immune response . In association with ACSS2, promotes the expression of genes involved in lysosome biogenesis and both autophagy upon glucose deprivation .

Transcription factor EB (Class E basic helix-loop-helix protein 35) (bHLHe35)

p19484

Human

WB,IHC,IF,ICC,IP,FC

WB 1:1000
IHC 1:200-1:500
IF 1:50
ICC 1:50
IP 1:20-1:50
FC 1:200-1:500

~53 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.