Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks , apoptosis and genotoxic stresses such as ionizing ultraviolet A light , thereby acting as a DNA damage sensor . Recognizes the substrate consensus sequence [ST]-Q . Phosphorylates 'Ser-139' of histone variant H2AX at double strand breaks , thereby regulating DNA damage response mechanism . Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor expressed on individual B-lymphocytes . After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele . Also involved in signal transduction and cell cycle control . May function as a tumor suppressor . Necessary for activation of ABL1 and SAPK . Phosphorylates DYRK2, CHEK2, p53/TP53, FBXW7, FANCD2, NFKBIA, BRCA1, CREBBP/CBP, RBBP8/CTIP, MRE11, nibrin , RAD50, RAD17, PELI1, TERF1, UFL1, RAD9, UBQLN4 and DCLRE1C . May play a role in vesicle and/or protein transport . Could play a role in T-cell development, gonad and neurological function . Binds DNA ends . Plays a role in replication-dependent histone mRNA degradation . Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation . Phosphorylates ATF2 which stimulates its function in DNA damage response . Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks . Phosphorylates TTC5/STRAP at 'Ser-203' in the cytoplasm in response to DNA damage, which promotes TTC5/STRAP nuclear localization . Also involved in pexophagy by mediating phosphorylation of PEX5: translocated to peroxisomes in response to reactive oxygen species , and catalyzes phosphorylation of PEX5, promoting PEX5 ubiquitination and induction of pexophagy .

Serine-protein kinase ATM (EC 2.7.11.1) (Ataxia telangiectasia mutated homolog) (A-T mutated homolog)

q62388

Human,Mouse,Rat

WB,IP

WB 1:1000
IP 1:100

~350 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.