Chromatin reader protein that specifically recognizes and binds histone H4 acetylated at 'Lys-5' and 'Lys-12' , thereby controlling gene expression and remodeling chromatin structures . Recruits transcription factors and coactivators to target gene sites, and activates RNA polymerase II machinery for transcriptional elongation . Plays a key role in genome compartmentalization via its association with CTCF and cohesin: recruited to chromatin by CTCF and promotes formation of topologically associating domains via its ability to bind acetylated histones, contributing to CTCF boundary formation and enhancer insulation . Also recognizes and binds acetylated non-histone proteins, such as STAT3 . Involved in inflammatory response by regulating differentiation of naive CD4 T-cells into T-helper Th17: recognizes and binds STAT3 acetylated at 'Lys-87', promoting STAT3 recruitment to chromatin . In addition to acetylated lysines, also recognizes and binds lysine residues on histones that are both methylated and acetylated on the same side chain to form N6-acetyl-N6-methyllysine , an epigenetic mark of active chromatin associated with increased transcriptional initiation . Specifically binds histone H4 acetyl-methylated at 'Lys-5' and 'Lys-12' .

Bromodomain-containing protein 2 (O27.1.1)

p25440

Human

WB

WB 1:1000

~89 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.