Guanine nucleotide-binding proteins function as transducers downstream of G protein-coupled receptors in numerous signaling cascades . The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state . Signaling by an activated GPCR promotes GDP release and GTP binding . The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal . Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins . Signaling is mediated via phospholipase C-beta-dependent inositol lipid hydrolysis for signal propagation: activates phospholipase C-beta: following GPCR activation, GNAQ activates PLC-beta , leading to production of diacylglycerol and inositol 1,4,5-trisphosphate . Required for platelet activation . Regulates B-cell selection and survival and is required to prevent B-cell-dependent autoimmunity . Regulates chemotaxis of BM-derived neutrophils and dendritic cells . Transduces FFAR4 signaling in response to long-chain fatty acids . Together with GNA11, required for heart development .

Guanine nucleotide-binding protein G(q) subunit alpha (EC 3.6.5.-) (Guanine nucleotide-binding protein alpha-q)

p50148

Human,Mouse,Rat

WB,IHC,IP,FC

WB 1:1000
IHC 1:200-1:1000
IP 1:20-1:50
FC 1:20-1:50

~43 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.