Catalyzes the reversible stereospecific interconversion of fumarate to L-malate . Experiments in other species have demonstrated that specific isoforms of this protein act in defined pathways and favor one direction over the other . ; [Isoform Mitochondrial]: Catalyzes the hydration of fumarate to L-malate in the tricarboxylic acid cycle to facilitate a transition step in the production of energy in the form of NADH. ; [Isoform Cytoplasmic]: Catalyzes the dehydration of L-malate to fumarate . Fumarate metabolism in the cytosol plays a role during urea cycle and arginine metabolism; fumarate being a by-product of the urea cycle and amino-acid catabolism . Also plays a role in DNA repair by promoting non-homologous end-joining . In response to DNA damage and phosphorylation by PRKDC, translocates to the nucleus and accumulates at DNA double-strand breaks : acts by catalyzing formation of fumarate, an inhibitor of KDM2B histone demethylase activity, resulting in enhanced dimethylation of histone H3 'Lys-36' .

Fumarate hydratase, mitochondrial (Fumarase) (HsFH) (EC 4.2.1.2)

p07954

Human,Mouse,Rat

WB,IHC,IF,ICC,IP,FC

WB 1:1000
IHC 1:200-1:2000
IF 1:500-1:1000
ICC 1:500-1:1000
IP 1:20-1:50
FC 1:200-1:500

~55 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.