Bifunctional modulator of guanine nucleotide-binding proteins . Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates guanine nucleotide-binding protein G alpha subunits . Also acts as a guanine nucleotide dissociation inhibitor for guanine nucleotide-binding protein G subunit alpha GNAS . Essential for cell migration . Interacts in complex with G alpha subunits with the EGFR receptor, retaining EGFR at the cell membrane following ligand stimulation and promoting EGFR signaling which triggers cell migration . Binding to Gi-alpha subunits displaces the beta and gamma subunits from the heterotrimeric G-protein complex which enhances phosphoinositide 3-kinase -dependent phosphorylation and kinase activity of AKT1/PKB . Phosphorylation of AKT1/PKB induces the phosphorylation of downstream effectors GSK3 and FOXO1/FKHR, and regulates DNA replication and cell proliferation . Binds in its tyrosine-phosphorylated form to the phosphatidylinositol 3-kinase regulatory subunit PIK3R1 which enables recruitment of PIK3R1 to the EGFR receptor, enhancing PI3K activity and cell migration . Plays a role as a key modulator of the AKT-mTOR signaling pathway, controlling the tempo of the process of newborn neuron integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation . Inhibition of G subunit alpha GNAS leads to reduced cellular levels of cAMP and suppression of cell proliferation . Essential for the integrity of the actin cytoskeleton . Required for formation of actin stress fibers and lamellipodia . May be involved in membrane sorting in the early endosome . Plays a role in ciliogenesis and cilium morphology and positioning and this may partly be through regulation of the localization of scaffolding protein CROCC/Rootletin .

Girdin (Akt phosphorylation enhancer) (APE) (Coiled-coil domain-containing protein 88A) (G alpha-interacting vesicle-associated protein) (GIV) (Girders of actin filament) (Hook-related protein 1) (HkRP1)

q3v6t2

Human,Mouse,Rat

WB,IF,ICC,IP,FC

WB 1:1000
IF 1:500-1:2000
ICC 1:500-1:2000
IP 1:50
FC 1:100

~217 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.