Molecular adapter required for selective macroautophagy by acting as a bridge between polyubiquitinated proteins and autophagosomes . Promotes the recruitment of ubiquitinated cargo proteins to autophagosomes via multiple domains that bridge proteins and organelles in different steps . SQSTM1 first mediates the assembly and removal of ubiquitinated proteins by undergoing liquid-liquid phase separation upon binding to ubiquitinated proteins via its UBA domain, leading to the formation of insoluble cytoplasmic inclusions, known as p62 bodies . SQSTM1 then interacts with ATG8 family proteins on autophagosomes via its LIR motif, leading to p62 body recruitment to autophagosomes, followed by autophagic clearance of ubiquitinated proteins . SQSTM1 is itself degraded along with its ubiquitinated cargos . Also required to recruit ubiquitinated proteins to PML bodies in the nucleus . Also involved in autophagy of peroxisomes in response to reactive oxygen species by acting as a bridge between ubiquitinated PEX5 receptor and autophagosomes . Acts as an activator of the NFE2L2/NRF2 pathway via interaction with KEAP1: interaction inactivates the BCR complex by sequestering the complex in inclusion bodies, promoting nuclear accumulation of NFE2L2/NRF2 and subsequent expression of cytoprotective genes . Promotes relocalization of 'Lys-63'-linked ubiquitinated STING1 to autophagosomes . Involved in endosome organization by retaining vesicles in the perinuclear cloud: following ubiquitination by RNF26, attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport . Sequesters tensin TNS2 into cytoplasmic puncta, promoting TNS2 ubiquitination and proteasomal degradation . May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor and interleukin-1 . May play a role in titin/TTN downstream signaling in muscle cells . Adapter that mediates the interaction between TRAF6 and CYLD .

Sequestosome-1 (EBI3-associated protein of 60 kDa) (EBIAP) (p60) (Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa) (Ubiquitin-binding protein p62) (p62)

q13501

Human, Mouse, Rat

WB,IF,ICC,IP,FC

WB 1:1000
IF 1:20
ICC 1:20
IP 1:20
FC 1:50

~48 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.