Serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGC family of protein kinases . Its targets include: protein kinase B , p70 ribosomal protein S6 kinase , p90 ribosomal protein S6 kinase , cyclic AMP-dependent protein kinase , protein kinase C , serum and glucocorticoid-inducible kinase , p21-activated kinase-1 , TSSK3, protein kinase PKN . Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage . Negatively regulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and the translocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta . Activates PPARG transcriptional activity and promotes adipocyte differentiation . Activates the NF-kappa-B pathway via phosphorylation of IKKB . The tyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II . Controls proliferation, survival, and growth of developing pancreatic cells . Participates in the regulation of Ca entry and Ca-activated K channels of mast cells . Essential for the motility of vascular endothelial cells and is involved in the regulation of their chemotaxis . Plays a critical role in cardiac homeostasis by serving as a dual effector for cell survival and beta-adrenergic response . Plays an important role during thymocyte development by regulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cell growth and proliferative responses . Provides negative feedback inhibition to toll-like receptor-mediated NF-kappa-B activation in macrophages . ; [Isoform 3]: Catalytically inactive.

3-phosphoinositide-dependent protein kinase 1 (hPDK1) (EC 2.7.11.1)

o15530

Human

WB,IP

WB 1:1000
IP 1:20-1:50

~64 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.