Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase signaling pathway . In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity . Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins . Loss of this interaction abrogates the acetylation required for replication initiation . Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1 . In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation . Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy . Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons . In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone . Phosphorylates several other substrates including heat shock factor protein 4 , the deacetylase SIRT1, ELK1, or the E3 ligase ITCH . Phosphorylates the CLOCK-BMAL1 heterodimer and plays a role in the regulation of the circadian clock . Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity . Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteasomal degradation . Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1 . In neurons, phosphorylates SYT4 which captures neuronal dense core vesicles at synapses . Phosphorylates EIF4ENIF1/4-ET in response to oxidative stress, promoting P-body assembly . Phosphorylates SIRT6 in response to oxidative stress, stimulating its mono-ADP-ribosyltransferase activity . Phosphorylates NLRP3, promoting assembly of the NLRP3 inflammasome . Phosphorylates ALKBH5 in response to reactive oxygen species , promoting ALKBH5 sumoylation and inactivation . ; JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.

Mitogen-activated protein kinase 8 (MAP kinase 8) (MAPK 8) (EC 2.7.11.24) (JNK-46) (Stress-activated protein kinase 1c) (SAPK1c) (Stress-activated protein kinase JNK1) (c-Jun N-terminal kinase 1)

p45983

Human,Mouse,Rat

WB,IF,ICC,IP,FC

WB 1:1000
IF 1:100-1:200
ICC 1:100-1:200
IP 1:50
FC 1:100-1:200

~49 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.