Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in -rich DNA regions . Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis . Inhibits SUV39H1 methyltransferase activity . Mediates ligand-dependent transcriptional activation by nuclear hormone receptors . Plays a critical role in maintaining genomic stability and cellular integrity following UV-induced genotoxic stress . Regulates the circadian expression of the core clock components NR1D1 and BMAL1 . Enhances the transcriptional repressor activity of NR1D1 through stabilization of NR1D1 protein levels by preventing its ubiquitination and subsequent degradation . Represses the ligand-dependent transcriptional activation function of ESR2 . Acts as a regulator of PCK1 expression and gluconeogenesis by a mechanism that involves, at least in part, both NR1D1 and SIRT1 . Negatively regulates the deacetylase activity of HDAC3 and can alter its subcellular localization . Positively regulates the beta-catenin pathway and is required for MCC-mediated repression of the beta-catenin pathway . Represses ligand-dependent transcriptional activation function of NR1H2 and NR1H3 and inhibits the interaction of SIRT1 with NR1H3 . Plays an important role in tumor suppression through p53/TP53 regulation; stabilizes p53/TP53 by affecting its interaction with ubiquitin ligase MDM2 . Represses the transcriptional activator activity of BRCA1 . Inhibits SIRT1 in a CHEK2 and PSEM3-dependent manner and inhibits the activity of CHEK2 in vitro .

Cell cycle and apoptosis regulator protein 2 (Cell division cycle and apoptosis regulator protein 2) (DBIRD complex subunit KIAA1967) (Deleted in breast cancer gene 1 protein) (DBC-1) (DBC.1) (NET35) (p30 DBC)

q8n163

Human

WB,IHC,IF,ICC,IP,FC

WB 1:1000
IHC 1:200-1:2000
IF 1:200-1:500
ICC 1:200-1:500
IP 1:20-1:50
FC 1:200-1:1000

~103 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.