Protein-arginine N-methyltransferase that catalyzes both the monomethylation and asymmetric dimethylation of the guanidino nitrogens of arginine residues in target proteins, and therefore falls into the group of type I methyltransferases . Catalyzes the asymmetric arginine dimethylation at multiple sites in the Arg/Gly-rich region of small ribosomal subunit protein uS5/RPS2 . Also appears to methylate other ribosomal proteins . May regulate retinoic acid synthesis and signaling by inhibiting ALDH1A1 retinal dehydrogenase activity . Contributes to methylation of histone H4 'Arg-3', a specific tag for epigenetic transcriptional activation . Mediates asymmetric arginine dimethylation of histone H4 'Arg-3' in the promoter region of miRNA miR-3648, to promote its transcription and osteogenesis .

Protein arginine N-methyltransferase 3 (EC 2.1.1.319) (Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3)

o60678

Human

WB,IHC,IF,ICC,IP

WB 1:2000-1:20000
IHC 1:100
IF 1:50
ICC 1:50
IP 1:20-1:50

~60 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.