Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling . Binding of antigen to the B-cell antigen receptor triggers signaling that ultimately leads to B-cell activation . After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C family members . PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK . BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways . Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors pathway . The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense . Especially, is a critical molecule in regulating TLR9 activation in splenic B-cells . Within the TLR pathway, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation . BTK also plays a critical role in transcription regulation . Induces the activity of NF-kappa-B, which is involved in regulating the expression of hundreds of genes . BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B . Acts as an activator of NLRP3 inflammasome assembly by mediating phosphorylation of NLRP3 . Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR . GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression . ARID3A and NFAT are other transcriptional target of BTK . BTK is required for the formation of functional ARID3A DNA-binding complexes . There is however no evidence that BTK itself binds directly to DNA . BTK has a dual role in the regulation of apoptosis . Plays a role in STING1-mediated induction of type I interferon response by phosphorylating DDX41 .

Tyrosine-protein kinase BTK (EC 2.7.10.2) (Agammaglobulinemia tyrosine kinase) (ATK) (B-cell progenitor kinase) (BPK) (Bruton tyrosine kinase)

q06187

Human,Mouse,Rat

WB,IHC,IF,ICC,IP,FC

WB 1:1000
IHC 1:500-1:2000
IF 1:100
ICC 1:100
IP 1:20-1:50
FC 1:200-1:500

~77 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.