Calcium-binding protein that acts as an adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes in response to calcium. Together with PDCD6, acts as a calcium-dependent adapter for the BCR complex, a complex involved in endoplasmic reticulum -Golgi transport by regulating the size of COPII coats . In response to cytosolic calcium increase, the heterodimer formed with PDCD6 interacts with, and bridges together the BCR complex and SEC31 , promoting monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification . Its role in the heterodimer formed with PDCD6 is however unclear: some evidence shows that PEF1 and PDCD6 work together and promote association between PDCD6 and SEC31 in presence of calcium . Other reports show that PEF1 dissociates from PDCD6 in presence of calcium, and may act as a negative regulator of PDCD6 . Also acts as a negative regulator of ER-Golgi transport; possibly by inhibiting interaction between PDCD6 and SEC31 .

Peflin (PEF protein with a long N-terminal hydrophobic domain) (Penta-EF hand domain-containing protein 1)

q9ubv8

Human,Mouse,Rat

WB,FC

WB 1:1000-1:5000
FC 1:10-1:100

~31 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.