Controls the reversible conversion of biologically active glucocorticoids such as cortisone to cortisol, and 11-dehydrocorticosterone to corticosterone in the presence of NADP . Participates in the corticosteroid receptor-mediated anti-inflammatory response, as well as metabolic and homeostatic processes . Plays a role in the secretion of aqueous humor in the eye, maintaining a normotensive, intraocular environment . Bidirectional in vitro, predominantly functions as a reductase in vivo, thereby increasing the concentration of active glucocorticoids . It has broad substrate specificity, besides glucocorticoids, it accepts other steroid and sterol substrates . Interconverts 7-oxo- and 7-hydroxy-neurosteroids such as 7-oxopregnenolone and 7beta-hydroxypregnenolone, 7-oxodehydroepiandrosterone and 7beta-hydroxydehydroepiandrosterone , among others . Catalyzes the stereo-specific conversion of the major dietary oxysterol, 7-ketocholesterol , into the more polar 7-beta-hydroxycholesterol metabolite . 7-oxocholesterol is one of the most important oxysterols, it participates in several events such as induction of apoptosis, accumulation in atherosclerotic lesions, lipid peroxidation, and induction of foam cell formation . Mediates the 7-oxo reduction of 7-oxolithocholate mainly to chenodeoxycholate, and to a lesser extent to ursodeoxycholate, both in its free form and when conjugated to glycine or taurine, providing a link between glucocorticoid activation and bile acid metabolism . Catalyzes the synthesis of 7-beta-25-dihydroxycholesterol from 7-oxo-25-hydroxycholesterol in vitro, which acts as a ligand for the G-protein-coupled receptor Epstein-Barr virus-induced gene 2 and may thereby regulate immune cell migration .

11-beta-hydroxysteroid dehydrogenase 1 (11-DH) (11-beta-HSD1) (EC 1.1.1.146) (7-oxosteroid reductase) (EC 1.1.1.201) (Corticosteroid 11-beta-dehydrogenase isozyme 1) (Short chain dehydrogenase/reductase family 26C member 1)

p28845

Human

WB,IHC,IF,ICC,FC

WB 1:2000-1:20000
IHC 1:200-1:2000
IF 1:50-1:100
ICC 1:50-1:100
FC 1:20

~33 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.