Catalyzes the hydrolysis of GTP and utilizes this energy to mediate vesicle scission and participates in many forms of endocytosis, such as clathrin-mediated endocytosis or synaptic vesicle endocytosis as well as rapid endocytosis . Associates to the membrane, through lipid binding, and self-assembles into rings and stacks of interconnected rings through oligomerization to form a helical polymer around the vesicle membrane leading to constriction of invaginated coated pits around their necks . Self-assembly of the helical polymer induces membrane tubules narrowing until the polymer reaches a length sufficient to trigger GTP hydrolysis . Depending on the curvature imposed on the tubules, membrane detachment from the helical polymer upon GTP hydrolysis can cause spontaneous hemifission followed by complete fission . May play a role in regulating early stages of clathrin-mediated endocytosis in non-neuronal cells through its activation by dephosphorylation via the signaling downstream of EGFR . Controls vesicle size at a step before fission, during formation of membrane pits, at hippocampal synapses . Controls plastic adaptation of the synaptic vesicle recycling machinery to high levels of activity . Mediates rapid endocytosis , a Ca-dependent and clathrin- and K-independent process in chromaffin cells . Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP . Through its interaction with DNAJC6, acts during the early steps of clathrin-coated vesicle formation .

Dynamin-1 (EC 3.6.5.5) (Dynamin) (Dynamin I)

q05193

Human,Mouse,Rat

WB,IHC

WB 1:1000
IHC 1:50

~98 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.