E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins . Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6 . Acts as a positive regulator of inflammatory response in microglia through activation of NF-kappa-B and MAP kinase . Mediates 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation . Conjugates 'Lys-63'-linked ubiquitin chains to the adapter protein ASC/PYCARD, which in turn is crucial for NLRP3 inflammasome activation . Mediates 'Lys-48'-linked polyubiquitination of RIPK3 leading to its subsequent proteasome-dependent degradation; preferentially recognizes and mediates the degradation of the 'Thr-182' phosphorylated form of RIPK3 . Negatively regulates necroptosis by reducing RIPK3 expression . Mediates 'Lys-63'-linked ubiquitination of RIPK1 . Following phosphorylation by ATM, catalyzes 'Lys-63'-linked ubiquitination of NBN, promoting DNA repair via homologous recombination . Negatively regulates activation of the metabolic mTORC1 signaling pathway by mediating 'Lys-63'-linked ubiquitination of mTORC1-inhibitory protein TSC1 and thereby promoting TSC1/TSC2 complex stability .

E3 ubiquitin-protein ligase pellino homolog 1 (Pellino-1) (EC 2.3.2.27) (Pellino-related intracellular-signaling molecule) (RING-type E3 ubiquitin transferase pellino homolog 1)

q96fa3

Human,Mouse,Rat

WB,IF,ICC,IP

WB 1:1000
IF 1:100
ICC 1:100
IP 1:20-1:50

~47 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.