NAD-dependent protein deacetylase, which deacetylates internal lysines on histone and alpha-tubulin as well as many other proteins such as key transcription factors . Participates in the modulation of multiple and diverse biological processes such as cell cycle control, genomic integrity, microtubule dynamics, cell differentiation, metabolic networks, and autophagy . Plays a major role in the control of cell cycle progression and genomic stability . Functions in the antephase checkpoint preventing precocious mitotic entry in response to microtubule stress agents, and hence allowing proper inheritance of chromosomes . Positively regulates the anaphase promoting complex/cyclosome ubiquitin ligase complex activity by deacetylating CDC20 and FZR1, then allowing progression through mitosis . Associates both with chromatin at transcriptional start sites and enhancers of active genes . Plays a role in cell cycle and chromatin compaction through epigenetic modulation of the regulation of histone H4 'Lys-20' methylation during early mitosis . Specifically deacetylates histone H4 at 'Lys-16' between the G2/M transition and metaphase enabling H4K20me1 deposition by KMT5A leading to ulterior levels of H4K20me2 and H4K20me3 deposition throughout cell cycle, and mitotic S-phase progression . Deacetylates KMT5A modulating KMT5A chromatin localization during the mitotic stress response . Also deacetylates histone H3 at 'Lys-57' during the mitotic G2/M transition . Upon bacterium Listeria monocytogenes infection, deacetylates 'Lys-18' of histone H3 in a receptor tyrosine kinase MET- and PI3K/Akt-dependent manner, thereby inhibiting transcriptional activity and promoting late stages of listeria infection . During oocyte meiosis progression, may deacetylate histone H4 at 'Lys-16' and alpha-tubulin, regulating spindle assembly and chromosome alignment by influencing microtubule dynamics and kinetochore function . Deacetylates histone H4 at 'Lys-16' at the VEGFA promoter and thereby contributes to regulate expression of VEGFA, a key regulator of angiogenesis . Deacetylates alpha-tubulin at 'Lys-40' and hence controls neuronal motility, oligodendroglial cell arbor projection processes and proliferation of non-neuronal cells . Phosphorylation at Ser-368 by a G1/S-specific cyclin E-CDK2 complex inactivates SIRT2-mediated alpha-tubulin deacetylation, negatively regulating cell adhesion, cell migration and neurite outgrowth during neuronal differentiation . Deacetylates PARD3 and participates in the regulation of Schwann cell peripheral myelination formation during early postnatal development and during postinjury remyelination . Involved in several cellular metabolic pathways . Plays a role in the regulation of blood glucose homeostasis by deacetylating and stabilizing phosphoenolpyruvate carboxykinase PCK1 activity in response to low nutrient availability . Acts as a key regulator in the pentose phosphate pathway by deacetylating and activating the glucose-6-phosphate G6PD enzyme, and therefore, stimulates the production of cytosolic NADPH to counteract oxidative damage . Maintains energy homeostasis in response to nutrient deprivation as well as energy expenditure by inhibiting adipogenesis and promoting lipolysis . Attenuates adipocyte differentiation by deacetylating and promoting FOXO1 interaction to PPARG and subsequent repression of PPARG-dependent transcriptional activity . Plays a role in the regulation of lysosome-mediated degradation of protein aggregates by autophagy in neuronal cells . Deacetylates FOXO1 in response to oxidative stress or serum deprivation, thereby negatively regulating FOXO1-mediated autophagy . Deacetylates a broad range of transcription factors and co-regulators regulating target gene expression. Deacetylates transcriptional factor FOXO3 stimulating the ubiquitin ligase SCF-mediated FOXO3 ubiquitination and degradation . Deacetylates HIF1A and therefore promotes HIF1A degradation and inhibition of HIF1A transcriptional activity in tumor cells in response to hypoxia . Deacetylates RELA in the cytoplasm inhibiting NF-kappaB-dependent transcription activation upon TNF-alpha stimulation . Inhibits transcriptional activation by deacetylating p53/TP53 and EP300 . Also deacetylates EIF5A . Functions as a negative regulator on oxidative stress-tolerance in response to anoxia-reoxygenation conditions . Plays a role as tumor suppressor . In addition to protein deacetylase activity, also has activity toward long-chain fatty acyl groups and mediates protein-lysine demyristoylation and depalmitoylation of target proteins, such as ARF6 and KRAS, thereby regulating their association with membranes . ; [Isoform 1]: Deacetylates EP300, alpha-tubulin and histone H3 and H4. ; [Isoform 2]: Deacetylates EP300, alpha-tubulin and histone H3 and H4. ; [Isoform 5]: Lacks deacetylation activity, at least toward known SIRT2 targets.

NAD-dependent protein deacetylase sirtuin-2 (EC 2.3.1.286) (NAD-dependent protein defatty-acylase sirtuin-2) (EC 2.3.1.-) (Regulatory protein SIR2 homolog 2) (SIR2-like protein 2)

q8ixj6

Human

WB

WB 1:1000

~44 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.