Adapter protein that plays a key role in innate immune response against fungi by forming signaling complexes downstream of C-type lectin receptors . CARD9-mediated signals are essential for antifungal immunity against a subset of fungi from the phylum Ascomycota . Transduces signals in myeloid cells downstream of C-type lectin receptors CLEC7A , CLEC6A and CLEC4E , which detect pathogen-associated molecular pattern metabolites , such as fungal carbohydrates, and trigger CARD9 activation . Upon activation, CARD9 homooligomerizes to form a nucleating helical template that recruits BCL10 via CARD-CARD interaction, thereby promoting polymerization of BCL10 and subsequent recruitment of MALT1: this leads to activation of NF-kappa-B and MAP kinase p38 pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines . CARD9 signaling in antigen-presenting cells links innate sensing of fungi to the activation of adaptive immunity and provides a cytokine milieu that induces the development and subsequent of interleukin 17-producing T helper cells . Also involved in activation of myeloid cells via classical ITAM-associated receptors and TLR: required for TLR-mediated activation of MAPK, while it is not required for TLR-induced activation of NF-kappa-B . CARD9 can also be engaged independently of BCL10: forms a complex with RASGRF1 downstream of C-type lectin receptors, which recruits and activates HRAS, leading to ERK activation and the production of cytokines . Acts as an important regulator of the intestinal commensal fungi component of the gut microbiota . Plays an essential role in antifungal immunity against dissemination of gut fungi: acts by promoting induction of antifungal IgG antibodies response in CX3CR1 macrophages to confer protection against disseminated C.albicans or C.auris infection . Also mediates immunity against other pathogens, such as certain bacteria, viruses and parasites; CARD9 signaling is however redundant with other innate immune responses . In response to L.monocytogenes infection, required for the production of inflammatory cytokines activated by intracellular peptidoglycan: acts by connecting NOD2 recognition of peptidoglycan to downstream activation of MAP kinases without activating NF-kappa-B .

Caspase recruitment domain-containing protein 9 (hCARD9)

q9h257

Human

WB,IP

WB 1:1000-1:5000
IP 1:20-1:50

~63 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.