Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca-mediated signals . Many of the substrates contain a PxIxIT motif and/or a LxVP motif . In response to increased Ca levels, dephosphorylates and activates phosphatase SSH1 which results in cofilin dephosphorylation . In response to increased Ca levels following mitochondrial depolarization, dephosphorylates DNM1L inducing DNM1L translocation to the mitochondrion . Positively regulates the CACNA1B/CAV2.2-mediated Ca release probability at hippocampal neuronal soma and synaptic terminals . Dephosphorylates heat shock protein HSPB1 . Dephosphorylates and activates transcription factor NFATC1 . In response to increased Ca levels, regulates NFAT-mediated transcription probably by dephosphorylating NFAT and promoting its nuclear translocation . Dephosphorylates and inactivates transcription factor ELK1 . Dephosphorylates DARPP32 . May dephosphorylate CRTC2 at 'Ser-171' resulting in CRTC2 dissociation from 14-3-3 proteins . Dephosphorylates transcription factor TFEB at 'Ser-211' following Coxsackievirus B3 infection, promoting nuclear translocation . Required for postnatal development of the nephrogenic zone and superficial glomeruli in the kidneys, cell cycle homeostasis in the nephrogenic zone, and ultimately normal kidney function . Plays a role in intracellular AQP2 processing and localization to the apical membrane in the kidney, may thereby be required for efficient kidney filtration . Required for secretion of salivary enzymes amylase, peroxidase, lysozyme and sialic acid via formation of secretory vesicles in the submandibular glands . Required for calcineurin activity and homosynaptic depotentiation in the hippocampus . Required for normal differentiation and survival of keratinocytes and therefore required for epidermis superstructure formation . Positively regulates osteoblastic bone formation, via promotion of osteoblast differentiation . Positively regulates osteoclast differentiation, potentially via NFATC1 signaling . May play a role in skeletal muscle fiber type specification, potentially via NFATC1 signaling . Negatively regulates MAP3K14/NIK signaling via inhibition of nuclear translocation of the transcription factors RELA and RELB . Required for antigen-specific T-cell proliferation response . Dephosphorylates KLHL3, promoting the interaction between KLHL3 and WNK4 and subsequent degradation of WNK4 . Negatively regulates SLC9A1 activity .

Protein phosphatase 3 catalytic subunit alpha (EC 3.1.3.16) (CAM-PRP catalytic subunit) (Calcineurin A alpha) (Calmodulin-dependent calcineurin A subunit alpha isoform) (CNA alpha) (Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform)

q08209

Human,Mouse,Rat

WB,FC

WB 1:1000-1:5000
FC 1:100

~59 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.