[Gasdermin-B]: Precursor of a pore-forming protein that acts as a downstream mediator of granzyme-mediated cell death . This form constitutes the precursor of the pore-forming protein: upon cleavage, the released N-terminal moiety binds to membranes and forms pores, triggering pyroptosis . Also acts as a regulator of epithelial cell repair independently of programmed cell death: translocates to the plasma membrane and promotes epithelial maintenance and repair by regulating PTK2/FAK-mediated phosphorylation of PDGFA . ; [Gasdermin-B, N-terminal]: Pore-forming protein produced by cleavage by granzyme A , which causes membrane permeabilization and pyroptosis in target cells of cytotoxic T and natural killer cells . Key downstream mediator of granzyme-mediated cell death: granzyme A , delivered to target cells from cytotoxic T- and NK-cells, specifically cleaves Gasdermin-B to generate this form . After cleavage, moves to the plasma membrane, homooligomerizes within the membrane and forms pores of 10-15 nanometers of inner diameter, triggering pyroptosis . The different isoforms recognize and bind different phospholipids on membranes, promoting cell death of different target cells . ; [Isoform 4]: Precursor of a pore-forming protein that acts as a downstream mediator of granzyme-mediated cell death and mediates pyroptosis . Following cleavage and activation by granzyme A , the N-terminal part binds to membrane inner leaflet lipids, homooligomerizes within the human plasma membrane and forms pores of 10-15 nanometers of inner diameter, triggering pyroptosis . Recognizes and binds membrane inner leaflet lipids of human cells, such as phosphatidylinositol 4-phosphate, phosphatidylinositol 5-phosphate, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol -bisphosphate, and more weakly to phosphatidic acid . Also binds sufatide, a component of the apical membrane of epithelial cells . ; [Isoform 6]: Precursor of a pore-forming protein that acts as a downstream mediator of granzyme-mediated cell death and mediates pyroptosis of human cells . Following cleavage and activation by granzyme A , the N-terminal part binds to membrane inner leaflet lipids, homooligomerizes within the human plasma membrane and forms pores of 10-15 nanometers of inner diameter, triggering pyroptosis . ; [Isoform 1]: Precursor of a pore-forming protein that acts as a downstream mediator of granzyme-mediated cell death and specifically mediates cell death of Gram-negative bacteria in response to infection . Following cleavage and activation by granzyme A , the N-terminal part recognizes and binds phospholipids found on Gram-negative bacterial membranes, such as lipid A and cariolipin, homooligomerizes within the bacterial membranes and forms pores, triggering pyroptosis followed by cell death . In contrast to isoform 4, does not bind to membrane inner leaflet lipids of host human cell, such as phosphatidylinositol 4-phosphate, phosphatidylinositol 5-phosphate, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol -bisphosphate . ; [Isoform 2]: Not able to trigger pyroptosis. ; [Isoform 3]: Not able to trigger pyroptosis.

Gasdermin-B (Gasdermin-like protein) [Cleaved into: Gasdermin-B, N-terminal (GSDMB-NT) (p30); Gasdermin-B, C-terminal (GSDMB-CT) (p16)]

q8tax9

Human

WB,IP,FC

WB 1:1000
IP 1:20-1:50
FC 1:200-1:500

~48 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.