Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 in response to low amino acid availability . Plays a role as an activator of the integrated stress response required for adaptation to amino acid starvation . EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha into a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, and thus to a reduced overall utilization of amino acids, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion . Binds uncharged tRNAs . Required for the translational induction of protein kinase PRKCH following amino acid starvation . Involved in cell cycle arrest by promoting cyclin D1 mRNA translation repression after the unfolded protein response pathway activation or cell cycle inhibitor CDKN1A/p21 mRNA translation activation in response to amino acid deprivation . Plays a role in the consolidation of synaptic plasticity, learning as well as formation of long-term memory . Plays a role in neurite outgrowth inhibition . Plays a proapoptotic role in response to glucose deprivation . Promotes global cellular protein synthesis repression in response to UV irradiation independently of the stress-activated protein kinase/c-Jun N-terminal kinase and p38 MAPK signaling pathways . Plays a role in the antiviral response against alphavirus infection; impairs early viral mRNA translation of the incoming genomic virus RNA, thus preventing alphavirus replication . ; Plays a role in modulating the adaptive immune response to yellow fever virus infection; promotes dendritic cells to initiate autophagy and antigene presentation to both CD4 and CD8 T-cells under amino acid starvation .

eIF-2-alpha kinase GCN2 (EC 2.7.11.1) (Eukaryotic translation initiation factor 2-alpha kinase 4) (GCN2-like protein)

q9p2k8

Human

WB

WB 1:1000

~187 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.