Chromatin binding factor that binds to DNA sequence specific sites and regulates the 3D structure of chromatin . Binds together strands of DNA, thus forming chromatin loops, and anchors DNA to cellular structures, such as the nuclear lamina . Defines the boundaries between active and heterochromatic DNA via binding to chromatin insulators, thereby preventing interaction between promoter and nearby enhancers and silencers . Plays a critical role in the epigenetic regulation . Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus . On the maternal allele, binding within the H19 imprinting control region mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2 . Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory . Regulates asynchronous replication of IGF2/H19 . Plays a critical role in gene silencing over considerable distances in the genome . Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones . Inversely, binding to target sites is prevented by CpG methylation . Plays an important role in chromatin remodeling . Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping . Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription . When bound to chromatin, it provides an anchor point for nucleosomes positioning . Seems to be essential for homologous X-chromosome pairing . May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation . May play a role in preventing the propagation of stable methylation at the escape genes from X-inactivation . Involved in sister chromatid cohesion . Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites . Plays a role in the recruitment of CENPE to the pericentromeric/centromeric regions of the chromosome during mitosis . Acts as a transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene . Also binds to the PLK and PIM1 promoters . Acts as a transcriptional activator of APP . Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression . Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription . Seems to act as tumor suppressor .

Transcriptional repressor CTCF (11-zinc finger protein) (CCCTC-binding factor) (CTCFL paralog)

p49711

Human,Mouse,Rat

WB,IHC,IF,ICC,FC

WB 1:1000-1:5000
IHC 1:200-1:1000
IF 1:200-1:500
ICC 1:200-1:500
FC 1:50

~83 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.