Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes . Can directly phosphorylate BAD and protects cells against apoptosis . Activated by interaction with CDC42 and RAC1 . Functions as a GTPase effector that links the Rho-related GTPases CDC42 and RAC1 to the JNK MAP kinase pathway . Phosphorylates and activates MAP2K1, and thereby mediates activation of downstream MAP kinases . Involved in the reorganization of the actin cytoskeleton, actin stress fibers and of focal adhesion complexes . Phosphorylates the tubulin chaperone TBCB and thereby plays a role in the regulation of microtubule biogenesis and organization of the tubulin cytoskeleton . Plays a role in the regulation of insulin secretion in response to elevated glucose levels . Part of a ternary complex that contains PAK1, DVL1 and MUSK that is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction . Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2 . Phosphorylates MYL9/MLC2 . Phosphorylates RAF1 at 'Ser-338' and 'Ser-339' resulting in: activation of RAF1, stimulation of RAF1 translocation to mitochondria, phosphorylation of BAD by RAF1, and RAF1 binding to BCL2 . Phosphorylates SNAI1 at 'Ser-246' promoting its transcriptional repressor activity by increasing its accumulation in the nucleus . In podocytes, promotes NR3C2 nuclear localization . Required for atypical chemokine receptor ACKR2-induced phosphorylation of LIMK1 and cofilin and for the up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation . In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3, maybe through CFL1 phosphorylation and inactivation . Plays a role in RUFY3-mediated facilitating gastric cancer cells migration and invasion . In response to DNA damage, phosphorylates MORC2 which activates its ATPase activity and facilitates chromatin remodeling . In neurons, plays a crucial role in regulating GABA receptor synaptic stability and hence GABAergic inhibitory synaptic transmission through its role in F-actin stabilization . In hippocampal neurons, necessary for the formation of dendritic spines and excitatory synapses; this function is dependent on kinase activity and may be exerted by the regulation of actomyosin contractility through the phosphorylation of myosin II regulatory light chain . Along with GIT1, positively regulates microtubule nucleation during interphase . Phosphorylates FXR1, promoting its localization to stress granules and activity . Phosphorylates ILK on 'Thr-173' and 'Ser-246', promoting nuclear export of ILK .

Serine/threonine-protein kinase PAK 1 (EC 2.7.11.1) (Alpha-PAK) (p21-activated kinase 1) (PAK-1) (p65-PAK)

q13153

Human,Mouse,Rat

WB,IHC,IF,ICC,IP,FC

WB 1:1000
IHC 1:50-1:200
IF 1:50-1:100
ICC 1:50-1:100
IP 1:50
FC 1:20

~61 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.