Key transcriptional regulator of type I interferon -dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses . Regulates the transcription of type I IFN genes and IFN-stimulated genes by binding to an interferon-stimulated response element in their promoters . Acts as a more potent activator of the IFN-beta gene than the IFN-alpha gene and plays a critical role in both the early and late phases of the IFNA/B gene induction . Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA , or toll-like receptor signaling, is phosphorylated by IKBKE and TBK1 kinases . This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein to form dsRNA-activated factor 1 , a complex which activates the transcription of the type I IFN and ISG genes . Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages . In response to Sendai virus infection, is recruited by TOMM70:HSP90AA1 to mitochondrion and forms an apoptosis complex TOMM70:HSP90AA1:IRF3:BAX inducing apoptosis . Key transcription factor regulating the IFN response during SARS-CoV-2 infection .

Interferon regulatory factor 3 (IRF-3)

q14653

Human

WB

WB 1:1000-1:5000

~48 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.