Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones , and some other non-histone substrates . Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events . Histone deacetylases act via the formation of large multiprotein complexes, such as N-Cor repressor complex, which activate the histone deacetylase activity . Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression . Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation . Contributes, together with XBP1 isoform 1, to the activation of NFE2L2-mediated HMOX1 transcription factor gene expression in a PIK/mTORC2/Akt-dependent signaling pathway leading to endothelial cell survival under disturbed flow/oxidative stress . Regulates both the transcriptional activation and repression phases of the circadian clock in a deacetylase activity-independent manner . During the activation phase, promotes the accumulation of ubiquitinated BMAL1 at the E-boxes and during the repression phase, blocks FBXL3-mediated CRY1/2 ubiquitination and promotes the interaction of CRY1 and BMAL1 . The NCOR1-HDAC3 complex regulates the circadian expression of the core clock gene BMAL1 and the genes involved in lipid metabolism in the liver . Also functions as a deacetylase for non-histone targets, such as KAT5, MEF2D, MAPK14, RARA and STAT3 . Serves as a corepressor of RARA, mediating its deacetylation and repression, leading to inhibition of RARE DNA element binding . In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response . In addition to protein deacetylase activity, also acts as a protein-lysine deacylase by recognizing other acyl groups: catalyzes removal of -butenoyl , lactoyl and 2-hydroxyisobutanoyl acyl groups from lysine residues, leading to protein decrotonylation, delactylation and de-2-hydroxyisobutyrylation, respectively . Catalyzes decrotonylation of MAPRE1/EB1 . Mediates delactylation NBN/NBS1, thereby inhibiting DNA double-strand breaks via homologous recombination .

Histone deacetylase 3 (HD3) (EC 3.5.1.98) (Protein deacetylase HDAC3) (EC 3.5.1.-) (Protein deacylase HDAC3) (EC 3.5.1.-) (RPD3-2) (SMAP45)

o15379

Human,Mouse,Rat

WB,IHC,IF,ICC,IP,FC

WB 1:1000-1:5000
IHC 1:50
IF 1:200-1:500
ICC 1:200-1:500
IP 1:20-1:50
FC 1:20

~49 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.