Essential antioxidant peroxidase that directly reduces phospholipid hydroperoxide even if they are incorporated in membranes and lipoproteins . Can also reduce fatty acid hydroperoxide, cholesterol hydroperoxide and thymine hydroperoxide . Plays a key role in protecting cells from oxidative damage by preventing membrane lipid peroxidation . Required to prevent cells from ferroptosis, a non-apoptotic cell death resulting from an iron-dependent accumulation of lipid reactive oxygen species . The presence of selenocysteine versus Cys at the active site is essential for life: it provides resistance to overoxidation and prevents cells against ferroptosis . The presence of Sec at the active site is also essential for the survival of a specific type of parvalbumin-positive interneurons, thereby preventing against fatal epileptic seizures . May be required to protect cells from the toxicity of ingested lipid hydroperoxides . Required for normal sperm development and male fertility . Essential for maturation and survival of photoreceptor cells . Plays a role in a primary T-cell response to viral and parasitic infection by protecting T-cells from ferroptosis and by supporting T-cell expansion . Plays a role of glutathione peroxidase in platelets in the arachidonic acid metabolism . Reduces hydroperoxy ester lipids formed by a 15-lipoxygenase that may play a role as down-regulator of the cellular 15-lipoxygenase pathway . Can also reduce small soluble hydroperoxides such as H2O2 and tert-butyl hydroperoxide . ; [Isoform Cytoplasmic]: Specifically able to suppress the production of leukotriene and prostaglandin in response to several stimuli by reducing fatty acid hydroperoxide. ; [Isoform Mitochondrial]: Specifically required to prevent mitochondrial cell death by mediating reduction of cardiolipin hydroperoxide . Also required for normal sperm development and male fertility . ; [Isoform Nuclear]: Required for male fertility by stabilizing the condensed chromatin in sperm nuclei .

Phospholipid hydroperoxide glutathione peroxidase GPX4 (PHGPx) (EC 1.11.1.12) (Glutathione peroxidase 4) (GPx-4) (GSHPx-4) (EC 1.11.1.9)

o70325

Human, Mouse, Rat

WB,IHC,IF,ICC,FC

WB 1:1000-1:5000
IHC 1:50-1:200
IF 1:200-1:500
ICC 1:200-1:500
FC 1:200

~23 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.