Guanine-nucleotide exchange factor for members of the Arf family of small GTPases involved in trafficking in the early secretory pathway; its GEF activity initiates the coating of nascent vesicles via the localized generation of activated ARFs through replacement of GDP with GTP. Recruitment to cis-Golgi membranes requires membrane association of Arf-GDP and can be regulated by ARF1, ARF3, ARF4 and ARF5. Involved in the recruitment of the COPI coat complex to the endoplasmic reticulum exit sites , and the endoplasmic reticulum-Golgi intermediate and cis-Golgi compartments which implicates ARF1 activation. Involved in COPI vesicle-dependent retrograde transport from the ERGIC and cis-Golgi compartments to the endoplasmic reticulum . Involved in the trans-Golgi network recruitment of GGA1, GGA2, GGA3, BIG1, BIG2, and the AP-1 adaptor protein complex related to chlathrin-dependent transport; the function requires its GEF activity . Has GEF activity towards ARF1 . Has in vitro GEF activity towards ARF5 . Involved in the processing of PSAP . Required for the assembly of the Golgi apparatus . The AMPK-phosphorylated form is involved in Golgi disassembly during mitotis and under stress conditions . May be involved in the COPI vesicle-dependent recruitment of PNPLA2 to lipid droplets; however, this function is under debate . In neutrophils, involved in G protein-coupled receptor -mediated chemotaxis und superoxide production. Proposed to be recruited by phosphatidylinositol-phosphates generated upon GPCR stimulation to the leading edge where it recruits and activates ARF1, and is involved in recruitment of GIT2 and the NADPH oxidase complex . Plays a role in maintaining mitochondrial morphology .

Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 (BFA-resistant GEF 1)

q92538

Human,Mouse,Rat

WB,IHC,IF,ICC,FC

WB 1:2000-1:20000
IHC 1:50-1:100
IF 1:200-1:500
ICC 1:200-1:500
FC 1:100-1:200

~207 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.