The catalytic subunit of the gastric H/K ATPase pump which transports H ions in exchange for K ions across the apical membrane of parietal cells. Uses ATP as an energy source to pump H ions to the gastric lumen while transporting K ion from the lumen into the cell . Remarkably generates a million-fold proton gradient across the gastric parietal cell membrane, acidifying the gastric juice down to pH 1 . Within a transport cycle, the transfer of a H ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation that shifts the pump conformation from inward-facing to outward-facing state . The release of the H ion in the stomach lumen is followed by binding of K ion converting the pump conformation back to the E1 state .

Potassium-transporting ATPase alpha chain 1 (EC 7.2.2.19) (Gastric H(+)/K(+) ATPase subunit alpha) (Proton pump)

p20648

Human,Rat

WB

WB 1:1000-1:5000

~115 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.