Protein kinase involved in the regulation of transcription . Member of the cyclin-dependent kinase pair complex, also called positive transcription elongation factor b , which facilitates the transition from abortive to productive elongation by phosphorylating the CTD of the large subunit of RNA polymerase II POLR2A, SUPT5H and RDBP . This complex is inactive when in the 7SK snRNP complex form . Phosphorylates EP300, MYOD1, RPB1/POLR2A and AR and the negative elongation factors DSIF and NELFE . Regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors . Promotes RNA synthesis in genetic programs for cell growth, differentiation and viral pathogenesis . P-TEFb is also involved in cotranscriptional histone modification, mRNA processing and mRNA export . Modulates a complex network of chromatin modifications including histone H2B monoubiquitination , H3 lysine 4 trimethylation and H3K36me3; integrates phosphorylation during transcription with chromatin modifications to control co-transcriptional histone mRNA processing . The CDK9/cyclin-K complex also has a kinase activity towards CTD of RNAP II and can substitute for CDK9/cyclin-T P-TEFb in vitro . Replication stress response protein; the CDK9/cyclin-K complex is required for genome integrity maintenance, by promoting cell cycle recovery from replication arrest and limiting single-stranded DNA amount in response to replication stress, thus reducing the breakdown of stalled replication forks and avoiding DNA damage . In addition, probable function in DNA repair of isoform 2 via interaction with KU70/XRCC6 . Promotes cardiac myocyte enlargement . RPB1/POLR2A phosphorylation on 'Ser-2' in CTD activates transcription . AR phosphorylation modulates AR transcription factor promoter selectivity and cell growth. DSIF and NELF phosphorylation promotes transcription by inhibiting their negative effect . The phosphorylation of MYOD1 enhances its transcriptional activity and thus promotes muscle differentiation . Catalyzes phosphorylation of KAT5, promoting KAT5 recruitment to chromatin and histone acetyltransferase activity .

Cyclin-dependent kinase 9 (EC 2.7.11.22) (EC 2.7.11.23) (C-2K) (Cell division cycle 2-like protein kinase 4) (Cell division protein kinase 9) (Serine/threonine-protein kinase PITALRE) (Tat-associated kinase complex catalytic subunit)

p50750

Human,Mouse,Rat

WB,IHC,IF,ICC,IP,FC

WB 1:1000-1:5000
IHC 1:100-1:200
IF 1:50
ICC 1:50
IP 1:20
FC 1:50-1:100

~43 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.