Involved in the initial and rate-limiting step of peroxisomal beta-oxidation of straight-chain saturated and unsaturated very-long-chain fatty acids. Catalyzes the desaturation of fatty acyl-CoAs such as palmitoyl-CoA to 2-trans-enoyl-CoAs -enoyl-CoAs) such as -hexadecenoyl-CoA, and donates electrons directly to molecular oxygen ), thereby producing hydrogen peroxide O). ; [Isoform 1]: Shows highest activity against medium-chain fatty acyl-CoAs. Shows optimum activity with a chain length of 10 carbons in vitro. ; [Isoform 2]: Is active against a much broader range of substrates and shows activity towards long-chain acyl-CoAs.

Peroxisomal acyl-coenzyme A oxidase 1 (AOX) (EC 1.3.3.6) (Palmitoyl-CoA oxidase) (Peroxisomal fatty acyl-CoA oxidase) (Straight-chain acyl-CoA oxidase) [Cleaved into: Peroxisomal acyl-CoA oxidase 1, A chain; Peroxisomal acyl-CoA oxidase 1, B chain; Peroxisomal acyl-CoA oxidase 1, C chain]

q9r0h0

Human,Mouse,Rat

WB,IF,ICC,IP,FC

WB 1:1000
IF 1:100-1:200
ICC 1:100-1:200
IP 1:20-1:50
FC 1:100

~75 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.