RNA reader protein, which recognizes and binds specific RNAs, thereby regulating RNA metabolic processes, such as pre-mRNA splicing, circular RNA formation, mRNA export, mRNA stability and/or translation . Involved in various cellular processes, such as mRNA storage into stress granules, apoptosis, lipid deposition, interferon response, glial cell fate and development . Binds to the 5'-NACUAAY-N-UAAY-3' RNA core sequence . Acts as a mRNA modification reader that specifically recognizes and binds mRNA transcripts modified by internal N-methylguanine . Promotes the formation of circular RNAs during the epithelial to mesenchymal transition and in cardiomyocytes: acts by binding to sites flanking circRNA-forming exons . CircRNAs are produced by back-splicing circularization of pre-mRNAs . Plays a central role in myelinization via 3 distinct mechanisms . First, acts by protecting and promoting stability of target mRNAs such as MBP, SIRT2 and CDKN1B, which promotes oligodendrocyte differentiation . Second, participates in mRNA transport by regulating the nuclear export of MBP mRNA . Finally, indirectly regulates mRNA splicing of MAG pre-mRNA during oligodendrocyte differentiation by acting as a negative regulator of MAG exon 12 alternative splicing: acts by binding to HNRNPA1 mRNA splicing factor, preventing its translation . Involved in microglia differentiation and remyelination by regulating microexon alternative splicing of the Rho GTPase pathway . Involved in macrophage differentiation: promotes monocyte differentiation by regulating pre-mRNA splicing in naive peripheral blood monocytes . Acts as an important regulator of muscle development: required for the contractile function of cardiomyocytes by regulating alternative splicing of cardiomyocyte transcripts . Acts as a negative regulator of thermogenesis by decreasing stability, nuclear export and translation of mRNAs encoding PPARGC1A and UCP1 . Also required for visceral endoderm function and blood vessel development . May also play a role in smooth muscle development . In addition to its RNA-binding activity, also acts as a nuclear transcription coactivator for SREBF2/SREBP2 . ; [Isoform QKI5]: Nuclear isoform that acts as an indirect regulator of mRNA splicing . Regulates mRNA splicing of MAG pre-mRNA by inhibiting translation of HNRNPA1 mRNA, thereby preventing MAG exon 12 alternative splicing . Involved in oligodendrocyte differentiation by promoting stabilization of SIRT2 mRNA . Acts as a negative regulator of the interferon response by binding to MAVS mRNA, downregulating its expression . Also inhibits the interferon response by binding to fibrinectin FN1 pre-mRNA, repressing EDA exon inclusion in FN1 . Delays macrophage differentiation by binding to CSF1R mRNA, promoting its degradation . In addition to its RNA-binding activity, also acts as a nuclear transcription coactivator for SREBF2/SREBP2, promoting SREBF2/SREBP2-dependent cholesterol biosynthesis . SREBF2/SREBP2-dependent cholesterol biosynthesis participates to myelinization and is required for eye lens transparency . ; [Isoform QKI6]: Cytosolic isoform that specifically recognizes and binds mRNA transcripts modified by internal N-methylguanine . Interaction with G3BP1 promotes localization of m7G-containing mRNAs into stress granules in response to stress, thereby suppressing their translation . Acts as a translational repressor for HNRNPA1 and GLI1 . Translation inhibition of HNRNPA1 during oligodendrocyte differentiation prevents inclusion of exon 12 in MAG pre-mRNA splicing . Involved in astrocyte differentiation by regulating translation of target mRNAs . ; [Isoform QKI7]: Cytosolic isoform that specifically recognizes and binds mRNA transcripts modified by internal N-methylguanine . Interaction with G3BP1 promotes localization of m7G-containing mRNAs into stress granules in response to stress, thereby suppressing their translation . Acts as a negative regulator of angiogenesis by binding to mRNAs encoding CDH5, NLGN1 and TNFAIP6, promoting their degradation . Can also induce apoptosis in the cytoplasm . Heterodimerization with other isoforms results in nuclear translocation of isoform QKI7 and suppression of apoptosis . Also binds some microRNAs: promotes stabilitation of miR-122 by mediating recruitment of poly RNA polymerase TENT2, leading to 3' adenylation and stabilization of miR-122 .

KH domain-containing RNA-binding protein QKI (Protein quaking) (Hqk) (HqkI)

q96pu8

Human,Mouse

WB,IF,ICC,IP

WB 1:1000-1:5000
IF 1:50-1:100
ICC 1:50-1:100
IP 1:10-1:100

~38 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.