Core component of multiple cullin-RING-based SCF E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. SCF complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins . In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme . The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and exchange of the substrate recognition component is mediated by TIP120A/CAND1 . The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component . SCF and SCF direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF directs ubiquitination of phosphorylated NFKBIA. SCF directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF and/or SCF direct ubiquitination of CEP68 . SCF directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF directs ubiquitination of CCNE1, NOTCH1 released notch intracellular domain , and probably PSEN1. SCF directs ubiquitination of GCM1. SCF directs ubiquitination of MYOD1. SCF directs ubiquitination of BIRC2 and DLGAP5. SCF directs ubiquitination of YBX1. SCF directs ubiquitination of BCL6 and DTL but does not seem to direct ubiquitination of TP53. SCF mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF directs ubiquitination of CCP110. SCF and SCF direct ubiquitination of CRY1 and CRY2. SCF directs ubiquitination of TTI1 and TELO2. SCF directs ubiquitination of BCL2. Neddylated CUL1-RBX1 ubiquitinates p53/TP53 recruited by Cul7-RING complex . SCF directs 'Lys-48'-linked ubiquitination of UBR2 in the T-cell receptor signaling pathway .

Cullin-1 (CUL-1)

q13616

Human,Mouse,Rat

WB,IF,ICC

WB 1:1000
IF 1:100-1:200
ICC 1:100-1:200

~90 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.