Adapter protein which non-covalently associates with activating receptors found on the surface of a variety of immune cells to mediate signaling and cell activation following ligand binding by the receptors . TYROBP is tyrosine-phosphorylated in the ITAM domain following ligand binding by the associated receptors which leads to activation of additional tyrosine kinases and subsequent cell activation . Also has an inhibitory role in some cells . Non-covalently associates with activating receptors of the CD300 family to mediate cell activation . Also mediates cell activation through association with activating receptors of the CD200R family . Required for neutrophil activation mediated by integrin . Required for the activation of myeloid cells mediated by the CLEC5A/MDL1 receptor . Associates with natural killer cell receptors such as KIR2DS2 and the KLRD1/KLRC2 heterodimer to mediate NK cell activation . Also enhances trafficking and cell surface expression of NK cell receptors KIR2DS1, KIR2DS2 and KIR2DS4 and ensures their stability at the cell surface . Associates with SIRPB1 to mediate activation of myeloid cells such as monocytes and dendritic cells . Associates with TREM1 to mediate activation of neutrophils and monocytes . Associates with TREM2 on monocyte-derived dendritic cells to mediate up-regulation of chemokine receptor CCR7 and dendritic cell maturation and survival . Association with TREM2 mediates cytokine-induced formation of multinucleated giant cells which are formed by the fusion of macrophages . Stabilizes the TREM2 C-terminal fragment produced by TREM2 ectodomain shedding which suppresses the release of pro-inflammatory cytokines . In microglia, required with TREM2 for phagocytosis of apoptotic neurons . Required with ITGAM/CD11B in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development . Promotes pro-inflammatory responses in microglia following nerve injury which accelerates degeneration of injured neurons . Positively regulates the expression of the IRAK3/IRAK-M kinase and IL10 production by liver dendritic cells and inhibits their T cell allostimulatory ability . Negatively regulates B cell proliferation . Required for CSF1-mediated osteoclast cytoskeletal organization . Positively regulates multinucleation during osteoclast development .

TYRO protein tyrosine kinase-binding protein (DNAX-activation protein 12) (Killer-activating receptor-associated protein) (KAR-associated protein)

o43914

Human

WB,IF,ICC,FC

WB 1:1000-1:5000
IF 1:200-1:500
ICC 1:200-1:500
FC 1:100

~13 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.