DNA repair protein involved in DNA non-homologous end joining ; it is required for double-strand break repair and VJ recombination and is also involved in telomere maintenance . Plays a key role in NHEJ by promoting the ligation of various mismatched and non-cohesive ends . Together with PAXX, collaborates with DNA polymerase lambda to promote joining of non-cohesive DNA ends . May act in concert with XRCC5-XRCC6 to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are non-complementary or partially complementary . In some studies, has been shown to associate with XRCC4 to form alternating helical filaments that bridge DNA and act like a bandage, holding together the broken DNA until it is repaired . Alternatively, it has also been shown that rather than forming filaments, a single NHEJ1 dimer interacts through both head domains with XRCC4 to promote the close alignment of DNA ends . The XRCC4-NHEJ1/XLF subcomplex binds to the DNA fragments of a DSB in a highly diffusive manner and robustly bridges two independent DNA molecules, holding the broken DNA fragments in close proximity to one other . The mobility of the bridges ensures that the ends remain accessible for further processing by other repair factors . Binds DNA in a length-dependent manner .

Non-homologous end-joining factor 1 (Protein cernunnos) (XRCC4-like factor)

q9h9q4

Human

WB,IHC,IF,ICC,FC

WB 1:2000-1:20000
IHC 1:100-1:200
IF 1:200-1:500
ICC 1:200-1:500
FC 1:50-1:100

~34 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.