Exhibits adenosine 5'-monophosphoramidase activity, hydrolyzing purine nucleotide phosphoramidates with a single phosphate group such as adenosine 5'monophosphoramidate to yield AMP and NH2 . Hydrolyzes adenosine 5'monophosphomorpholidate and guanosine 5'monophosphomorpholidate . Hydrolyzes lysyl-AMP ) generated by lysine tRNA ligase, as well as Met-AMP, His-AMP and Asp-AMP, lysyl-GMP ) and AMP-N-alanine methyl ester . Hydrolyzes 3-indolepropionic acyl-adenylate, tryptamine adenosine phosphoramidate monoester and other fluorogenic purine nucleoside tryptamine phosphoramidates in vitro . Can also convert adenosine 5'-O-phosphorothioate and guanosine 5'-O-phosphorothioate to the corresponding nucleoside 5'-O-phosphates with concomitant release of hydrogen sulfide . In addition, functions as scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and by the complex formed with MITF and CTNNB1 . Modulates p53/TP53 levels and p53/TP53-mediated apoptosis . Modulates proteasomal degradation of target proteins by the SCF E3 ubiquitin-protein ligase complex . Also exhibits SUMO-specific isopeptidase activity, deconjugating SUMO1 from RGS17 . Deconjugates SUMO1 from RANGAP1 .

Adenosine 5'-monophosphoramidase HINT1 (EC 3.9.1.-) (Desumoylating isopeptidase HINT1) (EC 3.4.22.-) (Histidine triad nucleotide-binding protein 1) (Protein kinase C inhibitor 1) (Protein kinase C-interacting protein 1) (PKCI-1)

p49773

Human,Mouse,Rat

WB,IHC,IF,ICC

WB 1:1000-1:5000
IHC 1:200-1:500
IF 1:500-1:1000
ICC 1:500-1:1000

~14 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.