Calcium sensor that plays a key role in processes such as endoplasmic reticulum -Golgi vesicular transport, endosomal biogenesis or membrane repair. Acts as an adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes in response to calcium: calcium-binding triggers exposure of apolar surface, promoting interaction with different sets of proteins thanks to 3 different hydrophobic pockets, leading to translocation to membranes . Involved in ER-Golgi transport by promoting the association between PDCD6IP and TSG101, thereby bridging together the ESCRT-III and ESCRT-I complexes . Together with PEF1, acts as a calcium-dependent adapter for the BCR complex, a complex involved in ER-Golgi transport by regulating the size of COPII coats . In response to cytosolic calcium increase, the heterodimer formed with PEF1 interacts with, and bridges together the BCR complex and SEC31 , promoting monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification . Involved in the regulation of the distribution and function of MCOLN1 in the endosomal pathway . Promotes localization and polymerization of TFG at endoplasmic reticulum exit site . Required for T-cell receptor-, Fas-, and glucocorticoid-induced apoptosis . May mediate Ca-regulated signals along the death pathway: interaction with DAPK1 can accelerate apoptotic cell death by increasing caspase-3 activity . Its role in apoptosis may however be indirect, as suggested by knockout experiments . May inhibit KDR/VEGFR2-dependent angiogenesis; the function involves inhibition of VEGF-induced phosphorylation of the Akt signaling pathway . In case of infection by HIV-1 virus, indirectly inhibits HIV-1 production by affecting viral Gag expression and distribution . ; [Isoform 2]: Has a lower Ca affinity than isoform 1 .

Programmed cell death protein 6 (Apoptosis-linked gene 2 protein homolog) (ALG-2)

o75340

Human,Mouse,Rat

WB,IHC

WB 1:1000
IHC 1:100-1:200

~22 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.