Phosphorylates a large number of substrates in the cytoplasm and the nucleus . Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA, SOX9 and VASP . Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis . RORA is activated by phosphorylation . Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts . Involved in chondrogenesis by mediating phosphorylation of SOX9 . Involved in the regulation of platelets in response to thrombin and collagen; maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B and I-kappa-B-alpha , but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP . Prevents the antiproliferative and anti-invasive effects of alpha-difluoromethylornithine in breast cancer cells when activated . RYR2 channel activity is potentiated by phosphorylation in presence of luminal Ca, leading to reduced amplitude and increased frequency of store overload-induced Ca release characterized by an increased rate of Ca release and propagation velocity of spontaneous Ca waves, despite reduced wave amplitude and resting cytosolic Ca . PSMC5/RPT6 activation by phosphorylation stimulates proteasome . Negatively regulates tight junctions in ovarian cancer cells via CLDN3 phosphorylation . NFKB1 phosphorylation promotes NF-kappa-B p50-p50 DNA binding . Required for phosphorylation of GLI transcription factors which inhibits them and prevents transcriptional activation of Hedgehog signaling pathway target genes . GLI transcription factor phosphorylation is inhibited by interaction of PRKACA with SMO which sequesters PRKACA at the cell membrane . Involved in embryonic development by down-regulating the Hedgehog signaling pathway that determines embryo pattern formation and morphogenesis most probably through the regulation of OFD1 in ciliogenesis . Prevents meiosis resumption in prophase-arrested oocytes via CDC25B inactivation by phosphorylation . May also regulate rapid eye movement sleep in the pedunculopontine tegmental . Phosphorylates APOBEC3G and AICDA . Phosphorylates HSF1; this phosphorylation promotes HSF1 nuclear localization and transcriptional activity upon heat shock . Acts as a negative regulator of mTORC1 by mediating phosphorylation of RPTOR . ; [Isoform 2]: Phosphorylates and activates ABL1 in sperm flagellum to promote spermatozoa capacitation.

cAMP-dependent protein kinase catalytic subunit alpha (PKA C-alpha) (EC 2.7.11.11)

p17612

Human,Mouse,Rat

WB,IHC,IF,ICC,IP,FC

WB 1:5000-1:50000
IHC 1:200-1:500
IF 1:100-1:200
ICC 1:100-1:200
IP 1:50
FC 1:50-1:100

~41 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.