Protein involved in various processes, such as stress granule formation and innate immunity . Plays an essential role in stress granule formation . Stress granules are membraneless compartments that store mRNAs and proteins, such as stalled translation pre-initiation complexes, in response to stress . Promotes formation of stress granules phase-separated membraneless compartment by undergoing liquid-liquid phase separation upon unfolded RNA-binding: functions as a molecular switch that triggers RNA-dependent LLPS in response to a rise in intracellular free RNA concentrations . Also acts as an ATP- and magnesium-dependent helicase: unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency . Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA . Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends . Plays an essential role in innate immunity by promoting CGAS and RIGI activity . Participates in the DNA-triggered cGAS/STING pathway by promoting the DNA binding and activation of CGAS . Triggers the condensation of cGAS, a process probably linked to the formation of membrane-less organelles . Also enhances RIGI-induced type I interferon production probably by helping RIGI at sensing pathogenic RNA . May also act as a phosphorylation-dependent sequence-specific endoribonuclease in vitro: Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR .

Ras GTPase-activating protein-binding protein 1 (G3BP-1) (EC 3.6.4.12) (EC 3.6.4.13) (ATP-dependent DNA helicase VIII) (hDH VIII) (GAP SH3 domain-binding protein 1)

q13283

Human

WB,IHC,IF,ICC,IP,FC

WB 1:2000-1:20000
IHC 1:50-1:100
IF 1:200-1:500
ICC 1:200-1:500
IP 1:20-1:50
FC 1:20

~53 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.