Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress . Binds to the insulin response element with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element with consensus sequence 5'-TT[G/A]TTTAC-3' . Activity suppressed by insulin . Main regulator of redox balance and osteoblast numbers and controls bone mass . Orchestrates the endocrine function of the skeleton in regulating glucose metabolism . Also acts as a key regulator of chondrogenic commitment of skeletal progenitor cells in response to lipid availability: when lipids levels are low, translocates to the nucleus and promotes expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation . Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity . Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP . Acts as an inhibitor of glucose sensing in pancreatic beta cells by acting as a transcription repressor and suppressing expression of PDX1 . In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes such as IGFBP1, G6PC1 and PCK1 . Also promotes gluconeogenesis by directly promoting expression of PPARGC1A and G6PC1 . Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and STK4/MST1 . Promotes neural cell death . Mediates insulin action on adipose tissue . Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake . Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells . Required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner . Mediates the function of MLIP in cardiomyocytes hypertrophy and cardiac remodeling . Positive regulator of apoptosis in cardiac smooth muscle cells as a result of its transcriptional activation of pro-apoptotic genes . Regulates endothelial cell viability and apoptosis in a PPIA/CYPA-dependent manner via transcription of CCL2 and BCL2L11 which are involved in EC chemotaxis and apoptosis .

Forkhead box protein O1 (Forkhead box protein O1A) (Forkhead in rhabdomyosarcoma)

q12778

Human,Mouse,Rat

WB,IHC

WB 1:1000-1:5000
IHC 1:200-1:500

~70 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.