Catalyzes the oxygenation of arachidonate -eicosatetraenoate) to 5-hydroperoxyeicosatetraenoate followed by the dehydration to 5,6- epoxyeicosatetraenoate , the first two steps in the biosynthesis of leukotrienes, which are potent mediators of inflammation . Also catalyzes the oxygenation of arachidonate into 8-hydroperoxyicosatetraenoate and 12-hydroperoxyicosatetraenoate . Displays lipoxin synthase activity being able to convert -HETE into a conjugate tetraene . Although arachidonate is the preferred substrate, this enzyme can also metabolize oxidized fatty acids derived from arachidonate such as -HETE, eicosapentaenoate such as - and -HEPE or docosahexaenoate which lead to the formation of specialized pro-resolving mediators lipoxin and resolvins E and D respectively, therefore it participates in anti-inflammatory responses . Oxidation of DHA directly inhibits endothelial cell proliferation and sprouting angiogenesis via peroxisome proliferator-activated receptor gamma . It does not catalyze the oxygenation of linoleic acid and does not convert -HETE to lipoxin isomers . In addition to inflammatory processes, it participates in dendritic cell migration, wound healing through an antioxidant mechanism based on heme oxygenase-1 regulation expression, monocyte adhesion to the endothelium via ITGAM expression on monocytes . Moreover, it helps establish an adaptive humoral immunity by regulating primary resting B cells and follicular helper T cells and participates in the CD40-induced production of reactive oxygen species after CD40 ligation in B cells through interaction with PIK3R1 that bridges ALOX5 with CD40 . May also play a role in glucose homeostasis, regulation of insulin secretion and palmitic acid-induced insulin resistance via AMPK . Can regulate bone mineralization and fat cell differentiation increases in induced pluripotent stem cells .

Polyunsaturated fatty acid 5-lipoxygenase (EC 1.13.11.-) (Arachidonate 5-lipoxygenase) (5-LO) (5-lipoxygenase) (EC 1.13.11.34)

p09917

Human,Rat

WB,IHC,IF,ICC,FC

WB 1:1000
IHC 1:100
IF 1:100
ICC 1:100
FC 1:100

~78 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.