Dual cyclooxygenase and peroxidase in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate -eicosatetraenoate, AA, C20:4), with a particular role in the inflammatory response . The cyclooxygenase activity oxygenates AA to the hydroperoxy endoperoxide prostaglandin G2 , and the peroxidase activity reduces PGG2 to the hydroxy endoperoxide prostaglandin H2 , the precursor of all 2-series prostaglandins and thromboxanes . This complex transformation is initiated by abstraction of hydrogen at carbon 13 , followed by insertion of molecular O2 to form the endoperoxide bridge between carbon 9 and 11 that defines prostaglandins. The insertion of a second molecule of O2 yields a hydroperoxy group in PGG2 that is then reduced to PGH2 by two electrons . Similarly catalyzes successive cyclooxygenation and peroxidation of dihomo-gamma-linoleate ) and eicosapentaenoate ) to corresponding PGH1 and PGH3, the precursors of 1- and 3-series prostaglandins . In an alternative pathway of prostanoid biosynthesis, converts 2-arachidonoyl lysophopholipids to prostanoid lysophopholipids, which are then hydrolyzed by intracellular phospholipases to release free prostanoids . Metabolizes 2-arachidonoyl glycerol yielding the glyceryl ester of PGH2, a process that can contribute to pain response . Generates lipid mediators from n-3 and n-6 polyunsaturated fatty acids via a lipoxygenase-type mechanism. Oxygenates PUFAs to hydroperoxy compounds and then reduces them to corresponding alcohols . Plays a role in the generation of resolution phase interaction products during both sterile and infectious inflammation . Metabolizes docosahexaenoate ) to 17R-HDHA, a precursor of the D-series resolvins . As a component of the biosynthetic pathway of E-series resolvins , converts eicosapentaenoate ) primarily to 18S-HEPE that is further metabolized by ALOX5 and LTA4H to generate 18S-RvE1 and 18S-RvE2 . In vascular endothelial cells, converts docosapentaenoate ) to 13R-HDPA, a precursor for 13-series resolvins shown to activate macrophage phagocytosis during bacterial infection . In activated leukocytes, contributes to oxygenation of hydroxyeicosatetraenoates to diHETES . Can also use linoleate -octadecadienoate, C18:2) as substrate and produce hydroxyoctadecadienoates in a regio- and stereospecific manner, being -HODE -hydroxy--octadecadienoate) and -HODE -hydroxy--octadecadienoate) its major products . During neuroinflammation, plays a role in neuronal secretion of specialized preresolving mediators 15R-lipoxin A4 that regulates phagocytic microglia .

Prostaglandin G/H synthase 2 (EC 1.14.99.1) (Cyclooxygenase-2) (COX-2) (PHS II) (Prostaglandin H2 synthase 2) (PGH synthase 2) (PGHS-2) (Prostaglandin-endoperoxide synthase 2)

p35354

Human, Rat, Mouse

WB,IHC,IF,ICC,IP

WB 1:1000
IHC 1:100-1:1000
IF 1:50-1:200
ICC 1:50-1:200
IP 1:20-1:100

~69 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.