Catalyzes the oxidative deamination of D-amino acids with broad substrate specificity . Required to catabolize D-amino acids synthesized endogenously, of gastrointestinal bacterial origin or obtained from the diet, and to use these as nutrients . Regulates the level of D-amino acid neurotransmitters in the brain, such as D-serine, a co-agonist of N-methyl D-aspartate receptors, and may modulate synaptic transmission . Catalyzes the first step of the racemization of D-DOPA to L-DOPA, for possible use in an alternative dopamine biosynthesis pathway . Also catalyzes the first step of the chiral inversion of N-nitro-D-arginine methyl ester to its L-enantiomer L-NNA that acts as a nitric oxide synthase inhibitor . The hydrogen peroxide produced in the reaction provides protection against microbial infection; it contributes to the oxidative killing activity of phagocytic leukocytes and protects against bacterial colonization of the small intestine . Enzyme secreted into the lumen of the intestine may not be catalytically active and could instead be proteolytically cleaved into peptides with antimicrobial activity . The hydrogen peroxide produced in the reaction may also play a role in promoting cellular senescence in response to DNA damage . Could act as a detoxifying agent which removes D-amino acids accumulated during aging .

D-amino-acid oxidase (DAAO) (DAMOX) (DAO) (EC 1.4.3.3)

p14920

Human

WB

WB 1:1000-1:5000

~40 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.