Functions both as NADH oxidoreductase and as regulator of apoptosis . In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway . Release into the cytoplasm is mediated upon binding to poly-ADP-ribose chains . The soluble form found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA . Binds to DNA in a sequence-independent manner . Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates caspase-7 to amplify apoptosis . Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells . In contrast, participates in normal mitochondrial metabolism. Plays an important role in the regulation of respiratory chain biogenesis by interacting with CHCHD4 and controlling CHCHD4 mitochondrial import . ; [Isoform 4]: Has NADH oxidoreductase activity. Does not induce nuclear apoptosis. ; [Isoform 5]: Pro-apoptotic isoform.

Apoptosis-inducing factor 1, mitochondrial (EC 1.6.99.-) (Programmed cell death protein 8)

o95831

Human, Mouse, Rat

WB,IHC,IF,ICC,IP,FC

WB 1:1000
IHC 1:100
IF 1:100
ICC 1:100
IP 1:20
FC 1:20

~67 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.